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Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8αα-BF2*0401

Yanjie Liu1, Rong Chen1, Mansoor Tariq1

  • 1Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.

Acta Crystallographica. Section F, Structural Biology Communications
|September 9, 2014
PubMed

In the process of antigen presentation, the MHCI-CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X-ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8αα (CD8αα-BF2*0401) are reported. This complex was verified by SDS-PAGE analysis of a CD8αα-BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, β2-microglobulin and CD8α, respectively. The crystal of CD8αα-BF2*0401 diffracted to 2.8 Å resolution and belonged to space group P21, with unit-cell parameters a = 90.6, b = 90.8, c = 94.9 Å, β = 98°. The Matthews coefficient and solvent content were calculated to be 2.88 Å(3) Da(-1) and ∼57.3%, respectively.

Keywords:
BF2*0401CD8ααchicken

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