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Experimental studies on the persistence in distilled water of certain conditional pathogenic fungi
Abstract:
The authors followed up the behaviour in distilled water of 8 fungus strains belonging to four species (Candida albicans, C. krusei, Geotrichum candidum and Rhodotorula) kept at various temperatures (i.e. +4 degrees, +18 degrees and 25 degrees C). The average and the maximum survival periods were found to be as follows: C. albicans--476/1138 days, C. krusei--411/1138 days, G. candidum--127/219 days, and R. rubra--139/226 days. All strains revealed periodically multiplication bursts of variable intensity depending on the kind of species and the temperature at which the cultures were maintained. These findings plead for the existence of cryptic periods of growth in fungi, too. Although, during the experiments, the variants occurred as concerns the morphological and cultural characteristics, sugar fermentation, sugar and nitrate assimilation, germ tube formation did not undergo any modifications. The pathogenic characteristics of C. albicans strains for rabbit were retained even after 1096 days maintenance in distilled water at 18 degrees C.
Insights
Fungal strains survived in distilled water for extended periods, with some exhibiting growth bursts. Pathogenicity in Candida albicans remained stable, suggesting cryptic growth phases in fungi.
Area of Science:
- Microbiology
- Mycology
Background:
- Fungi, including Candida albicans, are significant in clinical and industrial settings.
- Understanding fungal survival mechanisms is crucial for various applications.
Purpose of the Study:
- To investigate the long-term survival and behavior of selected fungal strains in distilled water.
- To assess the impact of temperature on fungal viability and characteristics.
Main Methods:
- Eight fungal strains from four species (Candida albicans, C. krusei, Geotrichum candidum, Rhodotorula) were maintained in distilled water.
- Cultures were kept at +4°C, +18°C, and 25°C.
- Survival periods, multiplication bursts, and phenotypic characteristics were monitored.
Main Results:
- Average survival ranged from 127 days (G. candidum) to 476 days (C. albicans). Maximum survival reached 1138 days for C. albicans and C. krusei.
- All strains showed periodic multiplication bursts, influenced by species and temperature.
- Pathogenic traits of C. albicans remained unchanged after prolonged storage.
Conclusions:
- Fungal strains can survive in distilled water for extended durations, indicating potential cryptic growth phases.
- Temperature influences fungal survival and growth patterns.
- Long-term storage in distilled water did not alter key characteristics like pathogenicity in C. albicans.