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Related Experiment Video

Updated: Apr 24, 2026

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Published on: March 1, 2012

Bluetongue virus capsid assembly and maturation.

Bjorn-Patrick Mohl¹, Polly Roy²

¹Department of Pathogen Molecular Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK. Bjorn.Mohl@lshtm.ac.uk.

|September 9, 2014

Summary

Bluetongue virus (BTV) maturation involves core assembly within inclusion bodies and subsequent addition of outer capsid proteins. This process is linked to the cell

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Area of Science:

Virology
Molecular Biology
Cell Biology

Background:

Viral maturation is a critical phase of the viral life cycle, often linked to egress.
Bluetongue virus (BTV), a well-studied orbivirus, provides a model for understanding viral maturation.
BTV is a non-enveloped virus with a double-stranded RNA genome and a multi-layered protein capsid.

Purpose of the Study:

To review and detail the intricate process of Bluetongue virus (BTV) maturation.
To elucidate the mechanisms of BTV egress and its connection to maturation.
To highlight the molecular insights gained from extensive in vitro and in vivo studies of BTV.

Main Methods:

Review of existing literature on BTV molecular studies.
Analysis of various in vitro and in vivo experimental assays.
Focus on the interplay between viral components and host cell machinery.

Main Results:

BTV core maturation occurs outside inclusion bodies after initial replication.
Outer capsid protein addition is coupled to a non-lytic exocytic pathway.
BTV utilizes the host's endosomal sorting complex required for trafficking (ESCRT) pathway for egress, mediated by a non-structural glycoprotein.

Conclusions:

BTV maturation is a complex process involving distinct intracellular stages.
Viral egress is closely coordinated with maturation, utilizing host cell pathways.
Detailed understanding of BTV maturation and egress offers insights into orbivirus replication.