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Metallothionein induction in rat hippocampal neurons in primary culture
P Thakran1, M P Leuschen, M Ebadi
1Department of Pediatrics, University of Nebraska College of Medicine, Omaha 68105.
In Vivo (Athens, Greece)
|May 1, 1989
Abstract:
Primary cultures of hippocampal neurons are important for the study of trace elements in epileptogenesis. We developed a model system for culturing hippocampal neurons on poly-L-lysine in Iscove's modification of Dulbecco's MEM (IMDM) supplemented with K+, D-glucose, glutamine, insulin, p-amino benzoic acid, transferrin, BSA, beta-estradiol, gentamycin, and fungizone. Neurons were identified by histochemical staining for cholinesterase. Zinc at concentrations of 10(-9) to 10(-6) M induced metallothionein in hippocampal neuronal cultures. Maximum metallothionein induction occurred after 48 hrs incubation with zinc.