Related Experiment Video
Updated: Apr 24, 2026

Author Spotlight: Establishing an Accurate Microhardness Testing Protocol for Craniofacial Tissues
Published on: April 26, 2024
The influence of acidogenic challenge on enamel microhardness around restorations
Anelise Fernandes Montagner1, Caroline Vargas Bittencourt2, Fabio Zovico M Soares3
1Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil. animontag@gmail.com.
Purpose:
The purpose of this study was to evaluate the influence of acidogenic challenge on microhardness of enamel adjacent to adhesive restorations in primary and permanent teeth.
Methods:
Occlusal cavities were prepared and immediately restored with two adhesive systems and a composite resin. The specimens were divided into eight groups, according to the type of tooth (primary or permanent), adhesive system (etch-and-rinse or self-etching), and treatment (control or acidogenic challenge). The cariogenic challenge groups were submitted to pH cycling for 10 days. The teeth were prepared for a cross-section microhardness test. Analysis of variance and Tukey's post hoc test were used to analyze the data (α=5 percent).
Results:
The adhesive systems did not influence the enamel microhardness (P<.27). Primary teeth were more susceptible to acidogenic challenge (P=.004). The microhardness values for control groups were statistically similar at different indentation depths (P>.05); however, in the acidogenic challenge groups, lower microhardness values were observed in superficial measurements (P<.05).
Conclusions:
Acidogenic challenge negatively influenced the microhardness of the enamel surface adjacent to adhesive restorations. Mineral loss was higher in primary teeth and on the enamel surface.
More Related Videos
08:12Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
07:42Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material
Published on: December 20, 2024
Related Concept Videos
Acid Attack on Concrete
The rate at which hydrogen...
Mixtures of Acids
A Mixture of a Strong Acid and a Weak Acid
In a mixture of a strong acid and a weak acid, the strong acid dissociates completely and becomes a source of almost all the hydronium ions...
Titration of a Weak Acid with a Strong Base