[Comparative analysis of isolation methods of DNA from rhizosphere soil for research of microbiocenosis]

The comparative analysis of different methods for the isolation of microbial DNA from ordinary black earth and dark-gray podzolic soil of soybean rhizosphere was carried out. All preparations of DNA which isolated from dark-gray podzolic soil after purification using highly dispersed silica (AppliChem, Germany) were suitable for 16S rRNA genes amplification with universal bacterial primers 11F i 1492R. For DNA extraction from ordinary black earth suitable for PCR DNA preparations were obtained using two of the six investigated methods. Isolation of DNA using a commercial Power Soil DNA Isolation Kit (MO BIO, USA) can get a small amount of DNA suitable for molecular studies without further purification procedures.