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Updated: Apr 24, 2026
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Published on: September 27, 2016
Dew inspired breathing-based detection of genetic point mutation visualized by naked eye
Liping Xie1, Tongzhou Wang2, Tianqi Huang2
11] Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China [2] Institute of Molecular Medicine, College of Life and Health Science, Northeastern University, Shenyang 110004, China.
Abstract:
A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing transduced the RCA-amplified variances in DNA contents into visible contrast. The image could be recorded by a cell phone for further or even remote analysis. This green assay offers a naked-eye-reading method potentially applied for point-of-care liver cancer diagnosis in resource-limited regions.
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