Additive effect of simultaneously varying respiratory frequency and tidal volume on respiratory sinus arrhythmia

Our aims were to assess, in healthy young females and males, the effects of the linear joint variation of respiratory frequency (RF) and tidal volume (VT) on the logarithmic transformation of high-frequency power of RR intervals (lnHF). ECG and VT were recorded from 18 females and 20 males during three visually guided 30-s breathing maneuvers: linearly increasing RF (RFLI) at constant VT; linearly increasing VT (VTLI) followed by decreasing VT (VTLD) at fixed RF, and RFLI and VTLI-VTLD combined. VT of females was 20% smaller. Instantaneous RF and lnHF were computed from the time-frequency distributions of respiratory series and RR intervals. LnHF-RF and lnHF-VT relations were similar between genders. LnHF and RR intervals control-maneuver differences during combined maneuver were approximately equal to the sum of those of the independent maneuvers. LnHF-RFLI relation showed strong negative correlations in separated and combined conditions, with steeper slope in the latter (p < 0.001). LnHF-VTLI and lnHF-VTLD relations presented, in the independent maneuvers, three combinations of slopes of different sign, all with hysteresis, and in the combined maneuver, strong correlations with negative slope for VTLI and positive slope for VTLD, steeper (p < 0.001) and with greater hysteresis (p < 0.001) than the independent ones. LnHF responses to our fast, non-fatiguing and non-steady-state breathing maneuvers are: similar between genders; consistent attenuation due to RFLI, whether applied alone or combined; ambiguous and with hysteresis to independent VTLI-VTLD variations; systematic greater attenuation during RFLI combined with VTLI-VTLD, equal to the sum of the independent effects, indicating that there is no interference between them.