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Metabolomics of AS-5 RBC supernatants following routine storage.

A D'Alessandro1, K C Hansen, C C Silliman

  • 1Department of Biochemistry and Molecular Genetics, School of Medicine University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.

Vox Sanguinis
|September 10, 2014
PubMed
Summary

Stored red blood cells (RBCs) show altered metabolism and accumulate inflammatory lipids by day 42. Supernatants from these aged RBCs may negatively impact recipients due to their unique metabolomics profile.

Keywords:
oxidative stressred blood cellstoragetransfusion medicine

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Area of Science:

  • Transfusion Medicine
  • Metabolomics
  • Biochemistry

Background:

  • The safety of stored red blood cells (RBCs) is debated, with evidence linking longer storage (>14 days) to increased patient morbidity and mortality.
  • The specific changes in RBCs during storage and their impact on recipients remain incompletely understood.

Purpose of the Study:

  • To investigate the metabolomics profile of the supernatant from RBCs stored in Optisol additive solution-5.
  • To determine if storage duration affects RBC metabolism and supernatant composition.

Main Methods:

  • Red blood cell units were prepared from healthy donors and stored.
  • Supernatant samples were collected on day 1 and day 42 of storage.
  • Mass spectrometry-based metabolomics was employed to analyze the samples.

Main Results:

  • RBC energy metabolism was progressively impaired by day 42.
  • Glutathione and NADPH homeostasis were altered during storage.
  • Oxidized, pro-inflammatory lipids accumulated in the supernatant by the end of the storage period.

Conclusions:

  • The supernatant of stored RBCs exhibits significant metabolic changes over time.
  • Accumulated metabolites and lipids in the supernatant may pose a risk to transfusion recipients.