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Prevalence, characterization and antibiotic resistance of Pasteurella multocida isolated from bovine respiratory

Hossein Jamali1, Mojtaba Rezagholipour2, Sepideh Fallah3

  • 1Biohealth Science Program, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Veterinary Journal (London, England : 1997)
|September 10, 2014
PubMed

Insights

This study investigated Pasteurella multocida in Iranian calves with respiratory infections, finding it common and often resistant to penicillin G and streptomycin. Characterization revealed specific serogroups and genetic markers in the isolated bacteria.

Area of Science:

  • Veterinary Microbiology
  • Bovine Respiratory Diseases
  • Antimicrobial Resistance

Background:

  • Bovine respiratory infection is a significant concern in dairy and beef farming.
  • Pasteurella multocida is a key pathogen implicated in calf respiratory disease.
  • Understanding its prevalence, characteristics, and resistance patterns is crucial for effective disease management.

Purpose of the Study:

  • To determine the prevalence of Pasteurella multocida in calves with respiratory infections in Iran.
  • To characterize the isolated Pasteurella multocida strains, including serogrouping and gene profiling.
  • To assess the antibiotic resistance patterns of Pasteurella multocida isolates.

Main Methods:

  • Isolation and identification of Pasteurella multocida from clinical samples of calves with respiratory signs.
  • Serogrouping of isolates using standard methods.
  • Detection of virulence-associated genes using molecular techniques.
  • Antimicrobial susceptibility testing against common antibiotics.

Main Results:

  • Pasteurella multocida was detected in 141 out of 169 (83.4%) cases.
  • Serogroup A was the most prevalent (126/141), followed by D (12/141) and B (3/141).
  • All isolates possessed specific genes (psl, ompH, oma87, fimA, ptfA, nanB, nanH), with high prevalence of hsf-2, pfhA, and tadD.
  • The highest resistance rates were observed for penicillin G (30.5%) and streptomycin (22%).

Conclusions:

  • Pasteurella multocida is highly prevalent in Iranian calves suffering from respiratory infections.
  • Specific serogroups and virulence genes are common in these isolates.
  • Significant resistance to penicillin G and streptomycin necessitates careful antibiotic selection for treatment.
Keywords:
Antibiotic resistanceBovine respiratory infectionPasteurella multocidaSerogroupingVirulence genes

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