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Linked scans of peptides and protein digests: amino acid sequence determination of components of complex mixtures
H R Morris1, A Chatterjee, M Panico
1Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
Rapid Communications in Mass Spectrometry : RCM
|April 1, 1989
Abstract:
Two-sector linked-scan analysis of an unpurified proteolytic digest of a pyruvate decarboxylase enzyme (60,000 Da) has allowed the discovery and assignment of an amino-terminal post-translational modification and processing event. A difference in amino acid sequence from that predicted by a recently published nucleotide sequence has also been found. These results illustrate both the use and considerable potential of linked-scan methods for the analysis of complex biopolymer mixtures.