Related Experiment Videos
Comparison of amino acid sequence analysis by electron ionization and negative-ion chemical ionization mass
H Reinhard1, R Lauber, U P Schlunegger
1Institute of Organic Chemistry, University of Bern, Switzerland.
Rapid Communications in Mass Spectrometry : RCM
|April 1, 1989
Abstract:
The positive electron ionization and negative chemical ionization mass spectra of 15 different derivatives of the tripeptide Phe-Ala-Leu have been compared. Total ion currents and ion currents of sequence-characterizing ions have been measured and compared. The negative-ion spectra, using 10% carbon dioxide in argon as moderator gas, proved to be simpler and contained more abundant sequence ions than the positive electron ionization spectra.