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Published on: January 19, 2019
Immune reconstitution from peripheral blood mononuclear cells inhibits lung carcinoma growth in NOD/SCID mice
Xiang Liu1, Huiling Li2, Jun Liu3
1Department of Cardiothoracic Surgery, The Second Hospital Affiliated to University of South China, Hengyang, Hunan 421001, P.R. China ; Department of Cardiothoracic Surgery, Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong 510120, P.R. China.
Abstract:
Drug resistance and immune deficiency are important factors for the poor prognosis of lung carcinoma. The present study explored the possible protective effect of immune reconstitution from peripheral blood mononuclear cells (PBMCs) on multi-drug-resistant human lung carcinoma Am1010 cells in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. The inoculated tumor fragments grew rapidly in the NOD/SCID mice. The growth was significantly inhibited by intraperitoneal injection of PBMCs. In the mice injected with PBMCs, numerous CD3+ and CD8+ cells, but less CD4+ cells, were found in spleen and tumor tissues. These data suggest that PBMC transplantation inhibits lung carcinoma progression via the reconstitution of the immune system, particularly of cytotoxic T lymphocytes.
Insights
Peripheral blood mononuclear cells (PBMCs) can inhibit lung carcinoma progression. PBMC transplantation in mice demonstrated immune system reconstitution, particularly cytotoxic T lymphocytes, improving prognosis.
Area of Science:
- Immunology
- Oncology
- Transplantation immunology
Background:
- Lung carcinoma prognosis is often poor due to drug resistance and immune deficiency.
- Multi-drug-resistant lung cancer presents a significant therapeutic challenge.
Purpose of the Study:
- To investigate the protective effect of immune reconstitution using peripheral blood mononuclear cells (PBMCs) on multi-drug-resistant lung carcinoma.
- To evaluate the efficacy of PBMC transplantation in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice models.
Main Methods:
- Inoculation of multi-drug-resistant human lung carcinoma Am1010 cells in NOD/SCID mice.
- Intraperitoneal injection of PBMCs to assess immune reconstitution and anti-tumor effects.
- Analysis of immune cell populations (CD3+, CD4+, CD8+) in spleen and tumor tissues.
Main Results:
- Tumor fragments grew rapidly in untreated NOD/SCID mice.
- PBMC injection significantly inhibited tumor growth.
- Increased CD3+ and CD8+ T cells, with decreased CD4+ T cells, observed in spleen and tumor tissues of PBMC-treated mice.
Conclusions:
- PBMC transplantation effectively inhibits lung carcinoma progression in an immunocompromised mouse model.
- Immune reconstitution, particularly the expansion of cytotoxic T lymphocytes, is crucial for the anti-tumor effect.
- This study highlights the potential of PBMC-based immunotherapy for drug-resistant lung cancer.
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