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Updated: Apr 24, 2026
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Isolation and characterization of 27 microsatellite markers for the endemic species Diplarche multiflora (Ericaceae)
Lin Zhang1, Zhi-Rong Zhang2, Lian-Ming Gao3
1Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China ; Graduate School of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Premise Of The Study:
Microsatellite markers from the genome of Diplarche multiflora were developed and characterized to investigate its genetic diversity and population structure. •
Methods And Results:
Twenty-seven microsatellite loci were isolated from the genome of D. multiflora using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Of these markers, 17 were polymorphic, and the number of alleles for the polymorphic microsatellite markers ranged from two to four, with an average of 2.2 per allele. The observed and expected heterozygosities varied from 0.0000 to 1.0000 and from 0.0000 to 0.7826, respectively. •
Conclusions:
These polymorphic microsatellite markers will be useful for population genetic studies and for assessing the genetic diversity of this alpine species.
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