Related Experiment Video
Updated: Sep 21, 2026
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
Published on: March 5, 2017
Development of microsatellite markers for Leucosceptrum japonicum and L. stellipilum (Lamiaceae)
1Botanical Gardens, Tohoku University, Aoba, Sendai 980-0862, Japan.
Premise Of The Study:
We developed 10 new primers for amplifying microsatellite loci for both Leucosceptrum japonicum and L. stellipilum to study the genetic structure of hybrid zones between these two species. •
Methods And Results:
We isolated DNA fragments containing microsatellites from an enriched library and designed the 10 primer pairs amplifying the fragments. The number of alleles per locus ranged from one to 11 in both L. japonicum and L. stellipilum. The observed heterozygosity ranged from 0.00 to 0.84 and 0.00 to 1.00 in L. japonicum and L. stellipilum, respectively. The expected heterozygosity ranged from 0.00 to 0.84 and 0.00 to 0.89 in L. japonicum and L. stellipilum, respectively. •
Conclusions:
These newly developed markers will be useful in investigating genetic structure and hybridization patterns in hybrid zones between L. japonicum and L. stellipilum.
More Related Videos
10:27A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
08:33Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
