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Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)
1Department of Plant Sciences, North Dakota State University Fargo, North Dakota 58108-6050 USA.
Premise Of The Study:
Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. •
Methods And Results:
Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. •
Conclusions:
The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris) demonstrates their potential for use in closely related species.
