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Cost-effective microsatellite markers for Banksia integrifolia (Proteaceae)
Mohammad Fatemi1, Gary J Houliston2, Azadeh Haddadchi1
1Ecosystem Management, University of New England, Armidale, New South Wales 2351, Australia.
Premise Of The Study:
Microsatellite markers were developed to assess the level of genetic variation and population structure in Banksia integrifolia, a widespread species endemic to eastern Australia. •
Methods And Results:
We used next-generation sequencing approaches to identify and develop 11 polymorphic microsatellite markers with perfect tri- and tetranucleotide repeats. We tested these markers with 71 specimens from three populations. Observed and expected heterozygosities ranged from 0.0 to 0.875 and 0.0 to 0.763, respectively. •
Conclusions:
The developed markers will be valuable for studies of the population structure, mating system, and selection of provenances for restoration projects involving B. integrifolia.
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