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Development and characterization of polymorphic microsatellite loci in Phellodendron amurense (Rutaceae)
Jing-Hua Yu1, Chang-Mei Chen1, Shi-Jie Han2
1State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
Premise Of The Study:
Microsatellite markers were developed for the rare species Phellodendron amurense to assess the genetic diversity and population structure of this plant. •
Methods And Results:
In total, 27 microsatellite markers were developed for P. amurense by using an enriched genomic library and hybridization; all of these primers successfully amplified DNA fragments in P. amurense. These markers were screened in 74 individuals from four populations in China; 15 loci were found to be polymorphic, with the number of alleles per locus ranging from one to nine. •
Conclusions:
The microsatellite markers developed here represent a useful tool for studying the population genetic structure of P. amurense and to inform toward the development of effective conservation programs for this species.
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