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Microsatellite primers in the lichen symbiotic alga Trebouxia decolorans (Trebouxiophyceae)
Francesco Dal Grande1, Andreas Beck2, Garima Singh3
1Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, 60325 Frankfurt am Main, Germany.
Premise Of The Study:
Polymorphic microsatellite markers were developed for the symbiotic green alga Trebouxia decolorans to study fine-scale population structure and clonal diversity. •
Methods And Results:
Using Illumina pyrosequencing, 20 microsatellite primer sets were developed for T. decolorans. The primer sets were tested on 43 individuals sampled from four subpopulations in Germany. The primers amplified di-, tri-, and tetranucleotide repeats with three to 15 alleles per locus, and the unbiased haploid diversity per locus ranged from 0.636 to 0.821. •
Conclusions:
The identified microsatellite markers will be useful to study the genetic diversity, dispersal, and reproductive mode of this common lichen photobiont.
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