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Updated: Apr 24, 2026
A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
Published on: July 11, 2025
Development of microsatellite loci for the riparian tree species Melaleuca argentea (Myrtaceae) using 454 sequencing
Paul G Nevill1, Anna Williams1, Siegfried Krauss1
1Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth 6005 Western Australia, Australia ; School of Plant Biology, University of Western Australia, Nedlands 6009 Western Australia, Australia.
Premise Of The Study:
Microsatellite primers were developed for Melaleuca argentea (Myrtaceae) to evaluate genetic diversity and population genetic structure of this broadly distributed northern Australian riparian tree species. •
Methods And Results:
454 GS-FLX shotgun sequencing was used to obtain 5860 sequences containing putative microsatellite motifs. Two multiplex PCRs were optimized to genotype 11 polymorphic microsatellite loci. These loci were screened for variation in individuals from two populations in the Pilbara region, northwestern Western Australia. Overall, observed heterozygosities ranged from 0.27 to 0.86 (mean: 0.52) and the number of alleles per locus ranged from two to 13 (average: 4.3). •
Conclusions:
These microsatellite loci will be useful in future studies of the evolutionary history and population and spatial genetic structure in M. argentea, and inform the development of seed sourcing strategies for the species.
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