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Published on: February 20, 2012
Development and characterization of microsatellite markers for Central American Begonia sect. Gireoudia (Begoniaceae)
Alex D Twyford1, Richard A Ennos2, Catherine A Kidner1
1Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, United Kingdom ; Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JH, United Kingdom.
Premise Of The Study:
Transcriptome sequence data were used to design microsatellite primers for two widespread Central American Begonia species, B. heracleifolia and B. nelumbiifolia, to investigate population structure and hybridization. •
Methods And Results:
The transcriptome from vegetative meristem tissue from the related B. plebeja was mined for microsatellite loci, and 31 primer pairs amplified in the target species. Fifteen primer pairs were combined in two multiplex PCR reactions, which amplified an average of four alleles per locus. •
Conclusions:
The markers developed will be a valuable genetic resource for medium-throughput genotyping of Central American species of Begonia sect. Gireoudia. A subset of these markers have perfect sequence matches to Asian B. venusta, and are promising for studies in other Begonia sections.
