Related Experiment Video
Updated: Apr 24, 2026
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Published on: January 22, 2018
Single-copy nuclear gene primers for Streptanthus and other Brassicaceae from genomic scans, published data, and ESTs
N Ivalú Cacho1, Sharon Y Strauss1
1Department of Evolution and Ecology, University of California, Davis, One Shields Avenue, Davis, California 95616-5294 USA.
Premise Of The Study:
We report 11 primer sets for nine single-copy nuclear genes in Streptanthus and other Thelypodieae (Brassicaceae) and their utility at tribal-level and species-level phylogenetics in this poorly resolved group. •
Methods And Results:
We selected regions based on a cross-referenced matrix of previous studies and public Brassica expressed sequence tags. To design primers, we used alignments of low-depth-coverage Illumina sequencing of genomic DNA for two species of Brassica mapped onto Arabidopsis thaliana. We report several primer combinations for five regions that consistently amplified a single band and yielded high-quality sequences for at least 70% of the species assayed, and for four additional regions whose utility might be clade specific. •
Conclusions:
Our primers will be useful in improving resolution at shallow depths across the Thelypodieae, and likely in other Brassicaceae.
