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Published on: September 2, 2019
High-throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae)
Hannah Matheny1, Joan Edwards1, Luana S Maroja1
1Department of Biology, Williams College, Williamstown, Massachusetts 01267 USA.
Premise Of The Study:
Twelve microsatellite markers were developed for Primula mistassinica, a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. •
Methods And Results:
We used Roche/454 high-throughput technology to sequence microsatellite-enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di-, tri-, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. •
Conclusions:
Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change.
