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Updated: Apr 24, 2026
13:03
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
10.2K
Primers for phylogeny reconstruction in Bignonieae (Bignoniaceae) using herbarium samples
Alexandre R Zuntini1, Luiz Henrique M Fonseca1, Lúcia G Lohmann1
1Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090, São Paulo, São Paulo, Brazil.
Applications in Plant Sciences
|September 10, 2014
Summary
New primers for Bignonieae enable phylogenetic studies using herbarium samples. This standardized method successfully amplifies DNA from old specimens, offering a valuable alternative for research.
Area of Science:
- Botany
- Molecular Phylogenetics
- Conservation Biology
Background:
- Phylogenetic studies of Bignonieae are crucial for understanding plant evolution.
- Accessing sufficient and high-quality DNA from herbarium samples can be challenging.
Purpose of the Study:
- To develop new primers for Bignonieae to facilitate phylogenetic studies.
- To enable the use of herbarium samples as a DNA source for phylogenetic analysis.
Main Methods:
- Designed internal primers targeting the plastid ndhF gene, the rpl32-trnL intergenic spacer region, and the nuclear gene PepC.
- Amplified DNA extracted from herbarium specimens using the newly developed primers.
Main Results:
- Successfully amplified DNA from herbarium samples up to 53 years old.
- Obtained high-quality DNA data from these aged herbarium materials.
- Demonstrated the efficacy of the new primers for phylogenetic analysis.
Conclusions:
- The developed primers and standardized methodology are effective for Bignonieae phylogenetics.
- Herbarium materials can serve as a reliable alternative DNA source for phylogenetic studies in this plant clade.
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