Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of a quantitative method for the enumeration of <i>Salmonella enterica</i> serovar Infantis from environmental poultry feces based on most probable number approach followed by confirmatory qPCR.

Frontiers in microbiology·2026
Same author

Transformation-Aware Molecular Networking for Interpretation of Untargeted LC-HRMS Data.

ACS measurement science au·2026
Same author

Unveiling a <i>Salmonella</i> Enteritidis Outbreak in an Italian Meat Rabbit Farm: Histopathological Features and Epidemiological Investigation.

Animals : an open access journal from MDPI·2025
Same author

Lipidic nanomedicines enhance Hinokitiol activity on human primary macrophages from Ferroportin disease patients.

International journal of pharmaceutics·2025
Same author

Chronic Myeloid Leukemia and the T315I <i>BCR::ABL1</i> Mutation.

International journal of molecular sciences·2025
Same author

Drug delivery systems for mitochondrial targeting.

Nanoscale·2025

Related Experiment Video

Updated: Apr 24, 2026

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.5K

Functionalization of liposomes: microscopical methods for preformulative screening.

Daniela Belletti1, Maria Angela Vandelli, Massimo Tonelli

  • 1Department of Life Sciences , University of Modena and Reggio Emilia, Via Campi 183, Modena , Italy and.

Journal of Liposome Research
|September 10, 2014
PubMed
Summary
This summary is machine-generated.

Surface modification of liposomes using pegylation and monoclonal antibody conjugation can be effectively evaluated. Combined transmission electron microscopy (TEM) and atomic force microscopy (AFM) confirm successful surface engineering for targeted drug delivery systems.

Keywords:
Anti-CD138 mAbimmunoliposomesnegative staining transmission electron microscopystealth liposomes“height images” atomic force microscopy“phase images” atomic force microscopy

More Related Videos

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
11:30

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

27.0K
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
08:44

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

6.6K

Related Experiment Videos

Last Updated: Apr 24, 2026

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

7.5K
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
11:30

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

27.0K
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
08:44

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

6.6K

Area of Science:

  • Pharmaceutical Technology
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Developing smart drug delivery systems to target specific sites is a key challenge in pharmaceutical technology.
  • Surface modification of nanocarriers like liposomes, through pegylation or ligand conjugation (e.g., monoclonal antibodies - mAb), enhances circulation time and cellular targeting.
  • Microscopy techniques are crucial for assessing liposome morphology, size, and surface modifications.

Purpose of the Study:

  • To evaluate the efficacy of liposome surface engineering processes.
  • To demonstrate the combined application of Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM) for analyzing liposomal surface modifications.
  • To validate surface modifications for targeted drug delivery applications.

Main Methods:

  • Preparation of liposomes with two distinct pegylation technologies.
  • Conjugation of monoclonal antibodies (mAbs) to the liposome surface.
  • Analysis of liposomal surface modifications using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM).

Main Results:

  • TEM and AFM provided complementary data on liposome morphology and size.
  • The combined microscopy approach successfully identified and characterized surface modifications, including pegylation and mAb conjugation.
  • The study confirmed the feasibility of evaluating surface engineering efficacy through integrated TEM and AFM analysis.

Conclusions:

  • Combined TEM and AFM analysis is a powerful tool for assessing the success of liposome surface engineering.
  • This approach validates modifications crucial for developing advanced nanocarriers for targeted drug delivery.
  • The findings support the use of these microscopy techniques in pharmaceutical nanotechnology research and development.