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Genome edited sheep and cattle.

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Genome editing tools like TALEN mRNA can now create gene-edited cattle and sheep by direct zygote injection. This advance offers new methods for agricultural improvement and biomedical research in livestock.

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Area of Science:

  • Animal genetics and genomics
  • Biotechnology and bioengineering
  • Livestock improvement

Background:

  • Genome editing tools offer precise manipulation of animal genomes.
  • Applications include enhancing disease resistance, productivity, and creating biomedical models in livestock.
  • Previous livestock genome editing via zygote injection was limited primarily to pigs.

Purpose of the Study:

  • To demonstrate the efficacy of TALEN mRNA zygote injection for genome editing in cattle and sheep.
  • To target the myostatin (MSTN) gene in both species.
  • To introduce an innovative method for producing gene-edited cattle using ovum pickup, in vitro fertilization, and zygote microinjection (OPU-IVF-ZM).

Main Methods:

  • Direct microinjection of TALEN mRNA into zygotes of cattle and sheep.
  • Targeting the myostatin (MSTN) gene in both species.
  • Implementing the OPU-IVF-ZM technique for generating gene-edited cattle.

Main Results:

  • Successful generation of gene-edited cattle and sheep using TALEN mRNA zygote injection.
  • Demonstrated targeting of the myostatin (MSTN) gene in both species.
  • Established OPU-IVF-ZM as a viable method for producing gene-edited cattle.

Conclusions:

  • TALEN mRNA zygote injection is an effective method for genome editing in cattle and sheep.
  • The OPU-IVF-ZM technique provides a practical alternative to somatic cell nuclear transfer for livestock genetic improvement.
  • This technology has significant implications for livestock breeding, disease resistance, and the development of biomedical models.