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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Thiol modified chitosan self-assembled monolayer platform for nucleic acid biosensor.

Maumita Das Mukherjee1, Pratima R Solanki, Gajjala Sumana

  • 1Amity Institute of Applied Sciences, Amity University, Noida, Uttar Pradesh, India.

Applied Biochemistry and Biotechnology
|September 11, 2014
PubMed
Summary

This study developed a novel nucleic acid biosensor using thiol-modified chitosan on a gold electrode for detecting Mycobacterium tuberculosis DNA. The biosensor demonstrates high sensitivity and specificity for target DNA detection.

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Area of Science:

  • Biomaterials Science
  • Biosensor Technology
  • Molecular Diagnostics

Background:

  • Chitosan is a biocompatible polymer with potential for biosensor applications.
  • Developing sensitive and specific nucleic acid biosensors is crucial for infectious disease diagnostics.
  • Surface modification of chitosan is necessary to enhance its electrochemical properties and facilitate probe immobilization.

Purpose of the Study:

  • To fabricate a novel nucleic acid biosensor using thiol-modified chitosan (SH-CHIT) on a gold electrode.
  • To investigate the electrochemical and biocompatibility properties of the SH-CHIT modified electrode.
  • To detect Mycobacterium tuberculosis DNA sequences with high sensitivity and specificity.

Main Methods:

  • Preparation of a self-assembled monolayer (SAM) of SH-CHIT on gold-coated glass plates.
  • Chemical modification of chitosan with thioglycolic acid (TGA) evidenced by FT-IR.
  • Immobilization of DNA probes using EDC/NHS chemistry.
  • Electrochemical and optical studies for DNA detection.

Main Results:

  • SH-CHIT modification enhanced the electrochemical behavior of the chitosan electrode.
  • The fabricated bioelectrode specifically detected complementary DNA sequences.
  • The biosensor achieved a sensitivity of 1.69 × 10⁻⁶ A μM⁻¹ for target DNA detection at 0.01 μM concentration.

Conclusions:

  • The SH-CHIT/Au bioelectrode is a promising platform for sensitive and specific nucleic acid detection.
  • This biosensor offers a potential tool for the rapid diagnosis of Mycobacterium tuberculosis infections.
  • The study highlights the utility of chemically modified chitosan in advanced biosensor development.