Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Those who need it the most get it the least: Age specific reciprocal effects between social support and mental strain.

Acta psychologica·2022

Same author

Bleeding and ischaemic events after first bleed in anticoagulated atrial fibrillation patients: risk and timing.

European heart journal·2022

Same author

Managers' Action-Guiding Mental Models towards Mental Health-Related Organizational Interventions-A Systematic Review of Qualitative Studies.

International journal of environmental research and public health·2022

Same author

Effective refractive error coverage in adults aged 50 years and older: estimates from population-based surveys in 61 countries.

The Lancet. Global health·2022

Same author

BCC-Cu nanoparticles: from a transient to a stable allotrope by tuning size and reaction conditions.

Physical chemistry chemical physics : PCCP·2022

Same author

Applying risk matrices for assessing the risk of psychosocial hazards at work.

Frontiers in public health·2022

Search research articles

Related Experiment Video

Updated: Apr 24, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Vesicular aptasensor for the detection of thrombin.

Andreas Müller¹, Burkhard König

¹Institut für Organische Chemie, Universität Regensburg, 93040 Regensburg, Germany. burkhard.koenig@ur.de.

Chemical Communications (Cambridge, England)

|September 11, 2014

Summary

Researchers created novel aptasensors using phospholipid vesicles and thrombin-binding aptamers. These sensors detect analytes by changes in reporter dye emissions upon binding to the vesicle surface.

Area of Science:

Biotechnology
Materials Science
Analytical Chemistry

Background:

Self-assembled phospholipid vesicles offer a versatile platform for biosensing applications.
Aptamers, particularly thrombin-binding aptamers, are valuable recognition elements in molecular detection.
Developing sensitive and specific biosensors is crucial for various diagnostic and research purposes.

Purpose of the Study:

To functionalize phospholipid vesicles with thrombin-binding aptamers for aptasensor development.
To establish a signaling mechanism for analyte binding based on reporter dye emission changes.
To create a novel aptasensor platform for detecting analytes at the vesicle surface.

Main Methods:

Phospholipid vesicles were self-assembled.

More Related Videos

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Published on: October 12, 2012

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research

Published on: March 29, 2024

Related Experiment Videos

Last Updated: Apr 24, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Published on: October 12, 2012

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research

Published on: March 29, 2024

Thrombin-binding aptamers were conjugated to the vesicle surface via a thiol-click reaction.

Membrane co-embedded reporter dyes were utilized to monitor analyte binding events.

Main Results:

Successful functionalization of phospholipid vesicles with thrombin-binding aptamers was achieved.
The aptasensors demonstrated signal generation through changes in reporter dye emission properties.
Analyte binding to the vesicle surface correlated with detectable changes in optical signals.

Conclusions:

The developed aptasensor platform based on functionalized phospholipid vesicles is effective for analyte detection.
The thiol-click reaction provides a robust method for aptamer immobilization.
Changes in reporter dye emission serve as a reliable readout for aptasensor activity.