Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Assembly of Cytoskeletal Filaments

Assembly of Cytoskeletal Filaments

Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Author Correction: Modular RNA motifs for orthogonal phase separated compartments.

Nature communications·2025

Same author

Electrochemical pan-variant detection of SARS-CoV-2 through host cell receptor-mimicking molecular recognition.

Biosensors & bioelectronics·2025

Same author

Modular DNA origami-based electrochemical detection of DNA and proteins.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Modular RNA motifs for orthogonal phase separated compartments.

Nature communications·2024

Same author

Co-transcriptional production of programmable RNA condensates and synthetic organelles.

Nature nanotechnology·2024

Same author

Molecular robotic agents that survey molecular landscapes for information retrieval.

Nature communications·2024

Search research articles

Related Experiment Video

Updated: Apr 24, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Self-assembly of two-dimensional DNA origami lattices using cation-controlled surface diffusion.

Sungwook Woo¹, Paul W K Rothemund²

¹Department of Bioengineering, California Institute of Technology, Pasadena, California 91125, USA.

Nature Communications

|September 11, 2014

Summary

Researchers developed a method to create large-scale DNA origami lattices for nanoelectronics. By controlling cation concentrations, they achieved over 90% incorporation of DNA rectangles into ordered 2D lattices, advancing nanodevice fabrication.

More Related Videos

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Related Experiment Videos

Last Updated: Apr 24, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Area of Science:

Nanotechnology
Materials Science
Biophysics

Background:

DNA origami enables nanoscale organization with high resolution.
Large-scale, ordered DNA nanostructures are crucial for applications like nanoelectronics.
Controlling DNA-nanoparticle interactions is key for higher-order assembly.

Purpose of the Study:

To achieve large-scale, two-dimensional (2D) lattice formation of DNA origami rectangles.
To investigate the role of cation concentration in controlling DNA origami self-assembly.
To establish a method for predictable higher-order assembly of DNA nanostructures.

Main Methods:

Utilized DNA origami rectangles as building blocks.
Manipulated surface diffusion by stepwise changes in cation concentrations on mica surfaces.
Quantified lattice formation based on the fractional surface density of divalent cations (ñ(s2)).

Main Results:

Achieved over 90% incorporation of DNA rectangles into 2D lattices within a specific range of ñ(s2) (0.04–0.1).
Demonstrated that ñ(s2) is the optimal parameter for controlling DNA origami lattice assembly.
Showcased the ability to guide higher-order assembly through physical understanding of DNA–surface interactions.

Conclusions:

Stepwise control of cation concentration, specifically ñ(s2), enables predictable self-assembly of DNA origami into large-scale 2D lattices.
This approach provides a pathway for fabricating large arrays of nanodevices for technological applications.
Understanding fundamental DNA–surface binding mechanisms is critical for advancing DNA nanostructure assembly.