Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bushen Yijing formula exerts chondroprotective effects in knee osteoarthritis via the ERα/PI3K/AKT signaling pathway: Insights from network pharmacology and experimental validation.

Tissue & cell·2026
Same author

[Study on the influencing factors of vitamin D level in female patients with knee osteoarthritis].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2026
Same author

Cell biomechanics on muscle atrophy: from intricate mechanisms to therapeutic frontiers.

Annals of medicine·2025
Same author

The Effect of Manipulation Under Anesthesia for Secondary Frozen Shoulder: A Randomized Controlled Trial.

Pain and therapy·2022
Same author

Self-Reported Weather Sensitivity is Associated with Clinical Symptoms and Structural Abnormalities in Patients with Knee Osteoarthritis: A Cross-Sectional Study.

Rheumatology and therapy·2021
Same author

Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury.

Aging·2020

Related Experiment Video

Updated: Apr 24, 2026

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
07:25

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Published on: July 7, 2022

2.9K

Mash1 efficiently reprograms rat astrocytes into neurons.

Daofang Ding1, Leqin Xu1, Hao Xu1

  • 1Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, China ; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Neural Regeneration Research
|September 11, 2014
PubMed
Summary
This summary is machine-generated.

Astrocytes can be reprogrammed into neurons using only Mash1. This study demonstrates Mash1

Keywords:
Brn2Mash-1astrocytesnerve regenerationneural regenerationneuronsplasmidreprogramretrovirusthe National Basic Research Program of China (973 Program)β-tubulin

More Related Videos

AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke
05:40

AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke

Published on: November 29, 2024

1.0K
Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
10:13

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Published on: August 12, 2014

13.1K

Related Experiment Videos

Last Updated: Apr 24, 2026

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
07:25

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Published on: July 7, 2022

2.9K
AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke
05:40

AAV Systems and Mouse Models for Investigating Ectopic Expression of Neurod1 in Transduced Cells at Subacute and Chronic Times Post-Ischemic Stroke

Published on: November 29, 2024

1.0K
Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
10:13

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Published on: August 12, 2014

13.1K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Stem Cell Research

Background:

  • Astrocytes are glial cells in the brain.
  • Reprogramming cells into neurons is a key area of research.
  • Previous studies showed Mash1 and Brn2 cooperate to reprogram fibroblasts.

Purpose of the Study:

  • To investigate if Mash1 alone can reprogram astrocytes into neurons.
  • To explore astrocyte reprogramming for potential therapeutic applications.

Main Methods:

  • Astrocytes from Sprague-Dawley rats were used.
  • A recombinant MSCV-MASH1 plasmid was transfected into astrocytes.
  • Cell morphology and beta-tubulin expression were analyzed.

Main Results:

  • Mash1 was not detectable in control astrocytes but expressed after transfection.
  • Transfected astrocytes exhibited neuronal morphology.
  • Beta-tubulin expression significantly increased in Mash1-expressing astrocytes.

Conclusions:

  • Mash1 alone can reprogram astrocytes into neurons.
  • This finding offers a potential new method for neuronal cell generation.