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Biochemical evidence that the DLA-B locus codes for a class II determinant expressed on all canine peripheral blood
I Doxiadis1, K Krumbacher, J J Neefjes
1Institute of Immunogenetics, University Hospital of Essen, FRG.
We have analyzed the products of the canine DLA-B gene locus by biochemical and serological methods. Due to its ubiquitous expression on lymphocytes this antigen series was thought to be of class I nature. However, by two-dimensional gel electrophoresis and lysostrip experiments we could unequivocally identify the DLA-B antigens as typical class II gene products. The serological polymorphism is caused by the beta-chain, whereas the alpha-chain appears invariant. The presence of these class II gene products on almost all lymphocytes in the dog is in sharp contrast to the numerous reports on their restricted expression in other mammalia such as man and mouse.
We have analyzed the products of the canine DLA-B gene locus by biochemical and serological methods. Due to its ubiquitous expression on lymphocytes this antigen series was thought to be of class I nature. However, by two-dimensional gel electrophoresis and lysostrip experiments we could unequivocally identify the DLA-B antigens as typical class II gene products. The serological polymorphism is caused by the beta-chain, whereas the alpha-chain appears invariant. The presence of these class II gene products on almost all lymphocytes in the dog is in sharp contrast to the numerous reports on their restricted expression in other mammalia such as man and mouse.