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Updated: Apr 24, 2026
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
Published on: April 29, 2020
In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii
Melek Bilgin1, Tuba Yıldırım2, Murat Hökelek3
1Microbiology Laboratory, Samsun Education and Research Hospital, Samsun, Turkey.
Objective:
Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture.
Study Design:
In-vitro study.
Material And Methods:
Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures.
Results:
The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation.
Conclusion:
These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain.
