Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A proposed structural model of endothelin.

M J Spinella1, S R Krystek, D H Peapus

  • 1Albany Medical College, NY 12208.

Peptide Research
|July 1, 1989
PubMed
Summary

This study proposes a structural model for endothelin, a vasoconstrictor peptide. The model reveals its amphipathic nature, with distinct hydrophilic and hydrophobic regions, influencing its behavior at interfaces.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cryo-EM structure of the human Kir7.1 channel reveals the molecular basis of snowflake vitreoretinal degeneration disease.

Communications biology·2026
Same author

Volatile anaesthetics modulate voltage-gated sodium channel function at a site directly linked to channel gating.

Nature communications·2026
Same author

Volatile anaesthetics modulate voltage-gated sodium channel function at a site critical for gating.

bioRxiv : the preprint server for biology·2024
Same author

Structural basis for the rescue of hyperexcitable cells by the amyotrophic lateral sclerosis drug Riluzole.

Nature communications·2024
Same author

DichroPipeline: A suite of online and downloadable tools and resources for protein circular dichroism spectroscopic data analyses, interpretations, and their interoperability with other bioinformatics tools and resources.

Protein science : a publication of the Protein Society·2023
Same author

DichroIDP: a method for analyses of intrinsically disordered proteins using circular dichroism spectroscopy.

Communications biology·2023

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Peptide Chemistry

Background:

  • Endothelin is a potent vasoconstrictor peptide.
  • Understanding its structure is crucial for its biological function.

Purpose of the Study:

  • To determine the hydropathic nature, secondary structure, and flexibility of endothelin.
  • To propose a testable structural model for endothelin.

Main Methods:

  • Computational modeling of endothelin structure.
  • Partitioning measurements of synthetic endothelin in an ether/water system.
  • Microcrystal growth observations at an organic-aqueous interface.

Main Results:

  • A structural model was proposed: a rigid, hydrophilic N-terminus with two turns and an extended, hydrophobic C-terminal sheet.
  • Endothelin demonstrated amphipathic properties, partitioning at the ether/water interface in a concentration- and surface area-dependent manner.
  • Synthetic endothelin microcrystals grew at the organic-aqueous interface, supporting the amphipathic model.

Conclusions:

  • The proposed model provides a framework for understanding endothelin's structure-activity relationship.
  • Endothelin's amphipathic nature is key to its interaction with biological membranes and its function.
  • Further studies can validate and refine this structural model.

Related Experiment Videos