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An auto-flocculation strategy for Chlorella vulgaris
1College of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, 350108, Fujian, China, yshen@fzu.edu.cn.
Biotechnology Letters
|September 12, 2014
Summary
Adding glycine to microalgae cultivation stimulates extracellular polymeric substances (EPS) secretion, enhancing auto-flocculation. This method optimizes biomass recovery and solid concentration for efficient microalgal harvesting.
Area of Science:
- Biotechnology
- Marine Biology
- Environmental Science
Background:
- Extracellular polymeric substances (EPS) are crucial for microalgal flocculation.
- Developing efficient microalgal harvesting techniques is vital for sustainable practices.
Purpose of the Study:
- To investigate an auto-flocculation strategy using glycine to enhance EPS secretion.
- To optimize conditions for microalgal solid concentration and biomass recovery.
Main Methods:
- Microalgae were cultivated with glycine addition to stimulate EPS production.
- Mixing time, light intensity, and glycine dosage were varied to determine optimal conditions.
- Bound EPS concentration and solid yield were measured.
Main Results:
- Bound EPS positively correlated with achieved solid concentration.
- Increased mixing time enhanced bound EPS secretion.
- Glycine's effect on EPS production was dependent on light intensity, with varying outcomes at different light levels.
- Optimal conditions yielded a maximum solid concentration of 21.2 g/L and 71% biomass recovery.
Conclusions:
- Glycine-induced auto-flocculation is an effective strategy for microalgal harvesting.
- Optimizing light intensity, mixing time, and glycine dosage is key to maximizing microalgal solid concentration and recovery rates.
