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Rapid determination of three alkaloids from Lotus Plumule in human serum using an HPLC-DAD method with a short
Hui Du1, Jing Ren1, Sicen Wang1
1Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, China; School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Abstract:
An HPLC-DAD with a short monolithic column method was used for rapid, sensitive and simultaneous determination of liensinine, isoliensinine and neferine in human serum. Chromatographic separation was achieved on an RP-HPLC chromolithic column (50mm×4.6mm i.d.), with a mobile phase of methanol-water-triethylamine-acetic acid (70:30:0.2:0.05), at a flow rate of 1mlmin(-1). The calibration curves of three alkaloids exhibit good linear relationship in the concentration range of 0.5-80μgml(-1). The limits of detection (LODs) of three alkaloids were 20, 20 and 25ngml(-1), respectively, and the separation time was 4min. A comparative study has been performed with a normal C18 packed column (150mm×4.6mm i.d., 5μm). The separation time was 16min and the LODs of three alkaloids were 56, 56 and 65ngml(-1), respectively.
