Bio-nanogate controlled enzymatic reaction for virus sensing

Area of Science:

• Nanotechnology
• Biosensors
• Analytical Chemistry

Background:

• Avian influenza viruses (AIV) pose a significant threat to global health.
• Accurate and rapid detection of AIV is crucial for disease control.
• Existing detection methods can be time-consuming or require complex procedures.

Purpose of the Study:

• To develop an aptamer-based bifunctional bio-nanogate for selective molecular response.
• To control enzymatic reactions for electrochemical measurements.
• To achieve sensitive, selective, rapid, and label-free detection of avian influenza virus (AIV) H5N1.

Main Methods:

• Preparation of a nanoporous gold film using a metallic corrosion method.
• Characterization of the gold film's purity using energy-dispersive X-ray spectroscopy (EDS).
• Optimization of the bio-nanogate biosensor's analytical parameters.

Main Results:

• The developed bio-nanogate successfully detected AIV H5N1 within 1 hour.
• Achieved a low detection limit of 2(-9) hemagglutination units (HAU).
• Demonstrated a linear relationship in the virus titer range of 2(-10)-2(2) HAU with no interference from other AIV subtypes.

Conclusions:

• The aptamer-based bio-nanogate provides a sensitive and selective platform for AIV H5N1 detection.
• The developed electrochemical biosensor enables rapid, label-free, and quantitative analysis.
• This approach holds potential for the detection of other viral pathogens.