Related Experiment Video
Updated: Apr 24, 2026
Isolation and Biophysical Study of Fruit Cuticles
Published on: March 30, 2012
Simultaneous purification of limonin, nomilin and isoobacunoic acid from pomelo fruit (Citrus grandis) segment
Yu Xiang1, Jinping Cao, Fenglei Luo
1Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang Univ, Zijingang Campus, Hangzhou, 310058, PR China.
Unlabelled:
A method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo (Citrus Grandis). This method includes 3 steps, removing most impurities by macroporous resin HZ-816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi-preparative HPLC. Naringin was partially purified as a by-product of this process using Sephadex LH-20. All limonoids purified through this method reached 95% purity. The purified limonin, nomilin and isoobacunoic acid were identified according to the retention time of the standard substances using HPLC and characteristic fragment ions of LC-MS/MS.
Practical Application:
A method of getting health care products limonoids from fruit processing by-products segments membrane of pomelo.
