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NANOG upregulates c-Jun oncogene expression through binding the c-Jun promoter
Yanli Lin1, Fuyin Xiong1, Yanrong Zhou1
1Laboratory of Cell Engineering, Beijing Institute of Biotechnology, Beijing, P.R. China.
Molecular Carcinogenesis
|September 13, 2014
Summary
NANOG protein directly activates c-Jun gene expression in cancer cells by binding to its promoter. This interaction suggests NANOG
Area of Science:
- Molecular Biology
- Cancer Research
- Gene Regulation
Background:
- NANOG is implicated in neoplastic processes, but its precise role in tumorigenesis is unclear.
- Understanding NANOG's molecular mechanisms is crucial for cancer research.
Purpose of the Study:
- To elucidate the molecular mechanism of NANOG in tumorigenesis.
- To identify NANOG's direct target genes and their role in cancer.
Main Methods:
- Forced expression and downregulation of NANOG in cell lines.
- Dual luciferase reporter assays to assess promoter activity.
- Electromobility shift assays and chromatin immunoprecipitation to confirm protein-DNA binding.
- GST pulldown and immunoprecipitation assays for protein-protein interaction.
Main Results:
- NANOG overexpression increased c-Jun expression; NANOG downregulation decreased c-Jun expression.
- NANOG directly binds the c-Jun promoter at an ATTA motif (-160 to -268 region).
- NANOG and c-Jun proteins directly interact, suggesting a co-activator role for c-Jun.
Conclusions:
- c-Jun is a direct transcriptional target of NANOG.
- c-Jun may function as a co-activator for NANOG in cancer cells.
- NANOG may promote carcinogenesis through c-Jun activation.
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