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Related Concept Videos

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

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The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
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Enzyme Kinetics01:19

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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
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Related Experiment Video

Updated: Apr 24, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Ink-jet printing an optimal multi-enzyme system.

Yifei Zhang1, Fengjiao Lyu, Jun Ge

  • 1Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, P. R. China. junge@mail.tsinghua.edu.cn liuzheng@mail.tsinghua.edu.cn.

Chemical Communications (Cambridge, England)
|September 13, 2014
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Summary

Ink-jet printing enables precise multi-enzyme systems construction. This innovation allows for accurate enzyme ratios and spatial distribution, leading to novel applications like glucose detection and enzyme-based codes.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Enzyme immobilization is crucial for developing stable and reusable biocatalytic systems.
  • Precise control over enzyme spatial distribution and ratios is challenging with traditional methods.

Purpose of the Study:

  • To develop a novel ink-jet printing method for constructing multi-enzyme systems.
  • To achieve precise control over enzyme ratios and two-dimensional distribution.
  • To demonstrate the application of these systems in sensing and information encoding.

Main Methods:

  • Utilized ink-jet printing technology to deposit multiple enzymes onto a substrate.
  • Employed preset 'color' values to precisely control the concentration and placement of each enzyme.
  • Developed multi-enzyme systems for specific applications, including glucose detection and 2D code creation.

Main Results:

  • Successfully constructed multi-enzyme systems with defined enzyme ratios and spatial arrangements.
  • Demonstrated the efficacy of the print-on-paper systems for sensitive glucose detection.
  • Engineered an enzyme-enabled two-dimensional code, showcasing information storage capabilities.

Conclusions:

  • Ink-jet printing offers a versatile and precise platform for fabricating advanced multi-enzyme systems.
  • The developed method facilitates the creation of functional biocatalytic materials for diverse applications.
  • This approach opens new avenues for enzyme-based sensors and data storage solutions.