[Study of immunomodulatory activity of progesterone in an experimental model of Jurkat cells]

Progesterone plays important the role in the regulation of the immune system during pregnancy. We investigated the influence of progesterone on the activity of T-lymphocytes in a model system of Jurkat cells. Jurkat cells were stimulated with 50 μg/ml of phytohemaglutinin A (PHA) at 370C for 5 minutes. Then, PHA was removed by centrifugation, the cells were washed and cultured for 24 hours alone or with progesterone (added to the incubation medium of Jurkat cells at a concentration of 0.07 and 0.7 μl). The effect of progesterone on the level of apoptosis and expression of cytokines in jointly incubated intact and PHA-stimulated Jurkat cells. It was revealed that progesteron did not affect the intensity of apoptosis and cytokine expression in intact, but dose-dependently promotes intensification of apoptosis (to 88%) and inhibits the cytotoxic activity of jointly incubated intact and PHA-stimulated Jurkat cells (ratio of IL-2/IL-10 decreased from 2.56 to 1.53).