Jove
Visualize
Contact Us

Related Experiment Videos

Diphosphonates inhibit human osteoblast secretion and proliferation.

M A Khokher1, P Dandona

  • 1Department of Chemical Pathology and Human Metabolism, Royal Free Hospital School of Medicine, London, United Kingdom.

Metabolism: Clinical and Experimental
|February 1, 1989
PubMed
Summary

Diphosphonates, including APD, clodronate, and didronel, directly inhibit human osteoblasts. This action on osteoblasts, alongside inhibiting bone resorption, explains their effectiveness in treating Paget

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A mixed anti-inflammatory and pro-inflammatory response associated with a high dose of corticosteroids.

Current molecular medicine·2014
Same author

Relationship between erythrocyte GLUT1 function and membrane glycation in type 2 diabetes.

British journal of biomedical science·2012
Same author

Effects of insulin and other antihyperglycaemic agents on lipid profiles of patients with diabetes.

Diabetes, obesity & metabolism·2011
Same author

Atypical β- and α(2)-adrenoceptor Activation, Dibutyryl cAMP and Iloprost Stimulate [(45)Ca(2+)] Uptake by Human Platelets.

Platelets·2010
Same author

Evidence for in vivo Platelet Activation Following the Injection of Conventional Unfractionated Heparin.

Platelets·2010
Same author

A practical guide to male hypogonadism in the primary care setting.

International journal of clinical practice·2010
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Biochemistry
  • Bone Biology
  • Pharmacology

Background:

  • Diphosphonates are beneficial in Paget's disease.
  • They suppress alkaline phosphatase activity.
  • Their direct effects on osteoblasts require investigation.

Purpose of the Study:

  • To investigate the in vitro action of three diphosphonates on human osteoblasts.
  • To determine if diphosphonates directly affect osteoblast function.
  • To understand the mechanism behind diphosphonates' efficacy in Paget's disease.

Main Methods:

  • Cultured human osteoblasts were treated with aminohydroxypropylidene diphosphonate (APD), clodronate, and didronel.
  • Alkaline phosphatase secretion was measured.
  • Osteoblast proliferation was assessed via [3H]thymidine uptake.

Related Experiment Videos

Main Results:

  • All tested diphosphonates inhibited basal alkaline phosphatase secretion.
  • Diphosphonates also inhibited 1,25(OH)2 cholecalciferol-stimulated alkaline phosphatase secretion.
  • Uptake of [3H]thymidine by osteoblasts was reduced by all three diphosphonates.

Conclusions:

  • Diphosphonates exert a direct inhibitory effect on human osteoblasts.
  • This direct action, combined with inhibition of bone resorption, underlies their therapeutic benefit in Paget's disease.
  • Further research into diphosphonate mechanisms on bone cells is warranted.