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Phylogenetic associations with drug-resistant Mycobacterium tuberculosis isolates in a paediatric population

J Arora1, Z Sidiq1, S Sharma2

  • 1Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, India.

The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
|September 14, 2014
PubMed

Insights

Drug resistance is high in pediatric tuberculosis (TB) patients, with Beijing and CAS1-Delhi strains driving active transmission. This highlights the need for targeted interventions against these resistant Mycobacterium tuberculosis strains.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Pediatric tuberculosis (TB) serves as an indicator of community disease transmission.
  • Understanding drug resistance and phylogenetic patterns in pediatric TB is crucial for public health.

Purpose of the Study:

  • To investigate drug resistance patterns of Mycobacterium tuberculosis complex strains from children.
  • To explore the phylogenetic associations of these strains.

Main Methods:

  • Analyzed 97 Mycobacterium tuberculosis complex strains from 111 pediatric TB patients (2009-2011).
  • Performed drug susceptibility testing (DST) against four first-line drugs.
  • Conducted spoligotyping to determine phylogenetic clusters.

Main Results:

  • 68% of strains were resistant to at least one drug; 56.7% were multidrug-resistant (isoniazid and rifampicin resistance).
  • The majority (90%) belonged to principal genetic group 1.
  • Predominant spoligotyping clusters were SIT1/Beijing (28 strains) and SIT26/CAS1-Delhi (27 strains).

Conclusions:

  • Beijing and CAS1-Delhi lineages are significantly involved in pediatric TB.
  • These clades likely play a major role in the ongoing active transmission of tuberculosis.
Abstract

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