Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.7K
Microbial Biosensors01:17

Microbial Biosensors

84
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
84
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

702
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
702
Methods to Assess Microbial Communities01:19

Methods to Assess Microbial Communities

55
Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
55
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

829
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
829
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early-pregnancy maternal metabolite signatures, genetic predisposition, and perinatal depressive symptom trajectories: A prospective birth cohort study.

Journal of affective disorders·2025
Same author

Laser capture microdissection-assisted gas chromatography-triple-quadruple mass spectrometry for spatial metabolic profiling of esophageal squamous cell carcinoma.

Journal of pharmaceutical and biomedical analysis·2025
Same author

Constructing Built-In Electric Field in Hierarchical-Flower Heterostructure for High-Performance Serum Metabolic Assay.

Analytical chemistry·2025
Same author

Delta 4-desaturase sphingolipid 2 enhances prostate cancer stem-like traits through phytoceramide-mediated PI3K-AKT signaling pathway.

Carcinogenesis·2025
Same author

MetCohort: Precise Feature Detection and Correspondence for Untargeted Metabolomics in Large-Scale Cohort Studies.

Analytical chemistry·2025
Same author

Nontargeted screening strategy of chemical residues in animal-derived foods based on endogenous metabolite global annotation and interquartile range filtering by ultrahigh-performance liquid chromatography-high-resolution mass spectrometry.

Journal of chromatography. A·2025

Related Experiment Video

Updated: Apr 24, 2026

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
08:09

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

8.2K

Mass-spectrometry-based microbial metabolomics: recent developments and applications.

Peng Gao1, Guowang Xu

  • 1Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Analytical and Bioanalytical Chemistry
|September 14, 2014
PubMed
Summary
This summary is machine-generated.

Microbial metabolomics, a key part of systems biology, uses analytical platforms to study metabolites. This review highlights advances in mass spectrometry-based methods for microbial metabolomic analysis and applications.

More Related Videos

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.3K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

21.9K

Related Experiment Videos

Last Updated: Apr 24, 2026

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
08:09

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

8.2K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.3K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

21.9K

Area of Science:

  • Microbiology
  • Systems Biology
  • Analytical Chemistry

Background:

  • Metabolomics is a vital omics technique for characterizing metabolomes.
  • It plays an indispensable role in modern systems biology.
  • Microbial metabolomics, including metabolic footprint and fingerprint analyses, aids microbiological research.

Purpose of the Study:

  • To review recent technical advances in mass spectrometry-based microbial metabolomic analysis.
  • To highlight the value and applications of modern microbial metabolomics.

Main Methods:

  • Summary of chromatography-mass-spectrometry-based microbial metabolomic analysis steps.
  • Includes sample collection, metabolite extraction, instrument analysis, and data analysis.
  • Discussion of applications in representative fields.

Main Results:

  • Provides an overview of mass spectrometry and nuclear magnetic resonance spectroscopy in metabolomics.
  • Summarizes key steps in chromatography-mass-spectrometry-based microbial metabolomic workflows.
  • Illustrates diverse applications of microbial metabolomics.

Conclusions:

  • Mass spectrometry-based microbial metabolomics is a powerful tool.
  • Recent technical advances enhance its analytical capabilities.
  • Microbial metabolomics offers significant value in various research areas.