Enhanced glucose tolerance in pancreatic-derived factor (PANDER) knockout C57BL/6 mice

Area of Science:

• Metabolic research
• Endocrinology
• Diabetes research

Background:

• Pancreatic-derived factor (PANDER) is implicated in glucose homeostasis and type 2 diabetes (T2D).
• Limited animal models exist to study PANDER's role in T2D.
• A well-characterized murine model is needed to investigate PANDER's function.

Purpose of the Study:

• To generate and characterize a PANDER knockout mouse model on a C57BL/6 background.
• To investigate PANDER's role in glycemic regulation and hepatic insulin signaling.

Main Methods:

• Created a pure-bred PANDER knockout C57BL/6 (PANKO-C57) mouse model.
• Phenotypically characterized PANKO-C57 mice for glycemic regulation and hepatic insulin signaling.
• Analyzed fasting and insulin-stimulated metabolic parameters, including glucose tolerance, insulin, C-peptide, leptin, hepatic PKB/Akt, AMPK, SREBP-1, triglyceride, and glycogen levels.

Main Results:

• PANKO-C57 mice exhibited enhanced glucose tolerance.
• Male PANKO-C57 mice showed decreased fasting insulin and C-peptide, with increased leptin.
• Hepatic insulin signaling was significantly increased in PANKO-C57 mice during fasting.
• Insulin stimulation led to increased hepatic triglyceride and glycogen in PANKO-C57 mice.

Conclusions:

• The PANKO-C57 mouse model is suitable for studying PANDER's function in metabolic states.
• This model provides a valuable tool for elucidating PANDER's biological role and potential involvement in T2D.