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A beta 1-integrin receptor for fibronectin in human kidney glomeruli
D Kerjaschki1, P P Ojha, M Susani
1Department of Pathological Anatomy, University of Vienna, Austria.
The American Journal of Pathology
|February 1, 1989
Summary
The fibronectin receptor (FNR), a beta 1-integrin, is present in human glomeruli. This receptor is found in both healthy kidneys and those affected by glomerular diseases.
Area of Science:
- Nephrology
- Cell Biology
- Molecular Biology
Background:
- The fibronectin receptor (FNR) is a heterodimeric glycoprotein.
- It shares a common beta 1-chain with other integrin family members.
- Integrins are crucial for cell adhesion processes.
Purpose of the Study:
- To investigate the presence and localization of FNR in human glomeruli.
- To determine if FNR distribution differs in glomerular diseases.
Main Methods:
- Preparation of membrane fractions from isolated human glomeruli.
- Fibronectin-affinity chromatography to isolate FNR.
- Immunoblotting and immunoelectron microscopy for detection and localization.
Main Results:
- Two proteins (120 and 140 kd) bound to fibronectin and were released by Arg-Gly-Asp-Ser.
- Antibodies against FNR recognized these proteins and labeled glomeruli.
- FNR was localized to cell membranes of mesangial, epithelial, and endothelial cells in normal and diseased kidneys.
Conclusions:
- A beta 1-integrin-related fibronectin receptor is present in human glomeruli.
- Its distribution is similar in normal and diseased states, suggesting a consistent role in glomerular structure or function.