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Published on: September 6, 2017
The existence and role of microchimerism after microtransplantion
Xuedong Sun1, Mei Guo2, Qiyun Sun2
1Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Sciences, Beijing Normal University, 19, Xinjiekou Outer Street, Haidian District, Beijing 100875, PR China; Department of Hematology and Transplantation, Affiliated Hospital of Academy of Military Medical Sciences, 8, Dongda Street, Fengtai District, Beijing 100071 PR China.
Aim:
To study microchimerism's role and function after microtransplantation and identify novel genetic markers for microchimerism detection.
Methods:
Analyzing microchimerisms from patients microtransplanted to determine the presence of GSTT1, GSTM1, SRY and other genetic markers by real-time PCR.
Results:
Microchimerism could be detected for a short time after microtransplantation simultaneously with hematopoietic recovery. In conclusion, microchimerism might accelerate hematopoietic recovery and GSTT1 and GSTM1 genes could be used as genetic markers to differentiate donor cells.
Discussion:
Microchimerism could exist for a short time after microtransplantation and appears to function in hematopoietic recovery. According to published reports, cytokines secreted from microchimerisms could be detected in recipients and exhibit some function on the host. Therefore, cytokines secreted from donor cells are hypothesized to accelerate hematopoietic recovery. The evidence to prove a longer existence for microchimerism is insufficient and needs supports by additional experiments; however, we cannot deny its existence just because of the limited sensitivity of methods.
