Binning metagenomic contigs by coverage and composition
Johannes Alneberg1, Brynjar Smári Bjarnason1, Ino de Bruijn2
11] KTH Royal Institute of Technology, Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Stockholm, Sweden. [2].
Nature Methods
|September 15, 2014
Abstract:
Shotgun sequencing enables the reconstruction of genomes from complex microbial communities, but because assembly does not reconstruct entire genomes, it is necessary to bin genome fragments. Here we present CONCOCT, a new algorithm that combines sequence composition and coverage across multiple samples, to automatically cluster contigs into genomes. We demonstrate high recall and precision on artificial as well as real human gut metagenome data sets.


