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Silica-based nanofibers for electrospun ultra-thin layer chromatography
Toni E Newsome1, Susan V Olesik1
1Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, United States.
Journal of Chromatography. A
|September 15, 2014
Summary
New silica-based nanofibers advance electrospun ultra-thin layer chromatography (E-UTLC). Calcined nanofibers offer broad mobile phase compatibility and highly efficient separations for amino acids, expanding E-UTLC capabilities.
Area of Science:
- Analytical Chemistry
- Materials Science
- Chromatography
Background:
- Electrospun ultra-thin layer chromatography (E-UTLC) utilizes nanofibrous stationary phases.
- Developing versatile stationary phases compatible with diverse mobile phases and analytes is crucial for E-UTLC advancement.
Purpose of the Study:
- To develop and evaluate novel silica-based nanofiber stationary phases for E-UTLC.
- To investigate the impact of post-spinning treatments (crosslinking, calcination) on nanofiber performance.
- To compare the separation efficiency of these novel phases with commercial silica TLC plates.
Main Methods:
- Composite silica/polymer nanofibers were fabricated using electrospinning.
- Stationary phases were prepared from as-spun, crosslinked, and calcined nanofibers.
- E-UTLC separations of laser dyes and amino acids were performed and analyzed.
Main Results:
- Calcined silica-based nanofibers demonstrated no limitations in mobile phase, analyte solvent, or visualization techniques.
- Highly efficient amino acid separations were achieved on calcined nanofiber plates with low plate heights (8.6 μm) and high plate numbers (1400).
- Aligned nanofibers reduced analysis time but increased spot width.
Conclusions:
- Calcined silica-based nanofibers represent a versatile and highly efficient stationary phase for E-UTLC.
- This development significantly broadens the scope of mobile phases, analyte solvents, and visualization techniques applicable to E-UTLC.
- Silica-based nanofibers offer a promising platform for next-generation E-UTLC applications.
Keywords:
Aligned nanofiberAmino acidElectrospinningPlanar chromatographySilica nanofiberUltra-thin layer chromatographyMore Related Videos
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