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Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

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Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
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Enzyme Inhibition01:30

Enzyme Inhibition

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Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
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Oxidation of Phenols to Quinones01:17

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In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
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Restriction Enzymes01:11

Restriction Enzymes

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Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
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Enzyme Kinetics01:19

Enzyme Kinetics

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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
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Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

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The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
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Updated: Apr 23, 2026

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
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Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

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Quorum quenching enzymes.

Susanne Fetzner1

  • 1Institute of Molecular Microbiology and Biotechnology, University of Muenster, Corrensstrasse 3, D-48149 Muenster, Germany.

Journal of Biotechnology
|September 16, 2014
PubMed
Summary
This summary is machine-generated.

Quorum quenching enzymes inactivate bacterial communication signals, offering new strategies to combat infections and biofouling. This research explores enzymatic degradation of signaling molecules for antivirulence and anti-biofouling applications.

Keywords:
AcylasesLactonasesOxidoreductasesQuorum quenchingQuorum sensing

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Area of Science:

  • Microbiology
  • Biochemistry
  • Biotechnology

Background:

  • Bacteria coordinate behaviors using quorum sensing (QS) signal molecules.
  • QS networks control virulence factors and biofilm formation in pathogens.
  • Interfering with QS offers potential antivirulence and anti-biofouling strategies.

Purpose of the Study:

  • To explore quorum quenching (QQ) as a method to disrupt bacterial cell-to-cell communication.
  • To review enzymes and approaches for signal inactivation in QS systems.
  • To assess the potential of QQ for antivirulence therapies and biofouling control.

Main Methods:

  • Enzymatic degradation or modification of QS signal molecules.
  • Investigation of lactonases, acylases, and oxidoreductases targeting N-acyl homoserine lactones (AHLs) and other signals.
  • Application of QQ enzymes or bacteria in biocontrol, probiotics, and biofouling prevention.

Main Results:

  • Quorum quenching enzymes are widespread in bacteria and eukaryotes.
  • Various enzymes actively degrade or modify QS signals like AHLs.
  • QQ strategies show promise in crop protection, aquaculture, and membrane bioreactors.

Conclusions:

  • Enzymatic inactivation of QS signals is a viable antivirulence and anti-biofouling strategy.
  • The discovery of diverse QQ enzymes opens avenues for novel antibacterial approaches.
  • Further research into QQ holds promise for developing innovative therapeutic and industrial applications.