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Expression and functional role of CD23 on T cells
R J Armitage1, L K Goff, P C Beverley
1I.C.R.F. Human Tumour Immunology Group, Courtauld Institute of Biochemistry, London, GB.
European Journal of Immunology
|January 1, 1989
Summary
Activated tonsil T cells, but not peripheral blood T cells, express CD23 antigen. CD23 monoclonal antibodies and CD23+ material enhance T cell proliferation, indicating a regulatory role in immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- T cells play a crucial role in adaptive immunity.
- CD23 is a known B cell marker, but its role in T cells is less understood.
Purpose of the Study:
- To investigate the expression and function of CD23 antigen on T cells.
- To determine the role of CD23 in T cell activation and proliferation.
Main Methods:
- Activation of tonsil and peripheral blood T cells with mitogens (TPA, PHA) and cytokines (IL-4).
- Detection of CD23 expression using monoclonal antibodies.
- Assessing T cell proliferation in response to CD23 stimulation.
Main Results:
- CD23 antigen is expressed on 10-15% of activated tonsil T cells, but not peripheral blood T cells.
- CD23 monoclonal antibodies and CD23+ material from B cell lines enhance T cell proliferation.
- Size analysis of CD23 molecule on T cells differs based on activation method.
Conclusions:
- CD23 is expressed on activated tonsil T cells and influences their proliferation.
- CD23 may play a regulatory role in T cell responses to mitogens.